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Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature ArchiMer
Boutet, Isabelle; Jollivet, Didier; Shillito, Bruce; Moraga, Dario; Tanguy, Arnaud.
Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected...
Tipo: Text Palavras-chave: Oyster crassostrea gigas; Heat shock protein; Mid atlantic ridge; Cytosolic malate dehydrogenases; Extracellular hemoglobins; Lipid peroxidation; Northeast pacific; Enzyme activities; Arginine kinase; Dual symbiosis.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00129/24014/21970.pdf
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Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by C-13-fingerprinting ArchiMer
Riou, V.; Bouillon, S.; Serrao Santos, R.; Dehairs, F.; Colaco, A..
Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of their energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assess their ability to assimilate CO2, CH4 and multi-carbon compounds, we performed experiments in aquaria using C-13-labeled NaHCO3 (in the presence of H2S), CH4 or amino-acids and traced the incorporation of C-13 into total and phospholipid fatty acids (tFA and PLFA, respectively). 14:0; 15:0; 16:0; 16:1(n - 7) c+t; 18:1(n - 13) c+t and (n - 7) c+t; 20:1(n - 7); 20:2(n - 9,15); 18:3(n - 7) and (n - 5,10,13) PLFA were labeled in the presence of...
Tipo: Text Palavras-chave: Mid atlantic ridge; Mussel bathymodiolus azoricus; Methanotrophic bacteria; Marine invertebrates; Diversity; Organic compounds; Lipid composition; Dual symbiosis; Methane; Biomarkers.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00207/31868/30275.pdf
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